Methods for Studying Site-Specific O-GlcNAc Modifications: Successes, Limitations, and Important Future Goals

, each with their own respective strengths and shortcomings. Together, they comprise a potent chemical biology toolbox for the analysis of O-GlcNAcylation (and, in theory, other post-translational modifications) while highlighting the need and space for more facile, generalizable, and biologically authentic techniques.