血浆糖原
生物化学
多不饱和脂肪酸
胆碱
缺氧(环境)
脂肪酸
花生四烯酸
生物
细胞内
化学
磷脂
氧气
膜
有机化学
酶
作者
Keiko Tamiya‐Koizumi,Yurika Otoki,Kiyotaka Nakagawa,Reiji Kannagi,Naoki Mizutani,Motoshi Suzuki,Mamoru Kyogashima,Soichiro Iwaki,Mineyoshi Aoyama,Takashi Murate,Kazuyuki Kitatani,Tetsurō Kuga,Yasuyoshi Mizutani,Akira Tokumura
标识
DOI:10.1016/j.bbrc.2022.04.061
摘要
Plasmalogen localized in the raft of mammalian cell membranes plays a role in the storage of polyunsaturated fatty acid (PUFA), and exists to a higher extent in malignant cells that survive, and even grow in hypoxic conditions. The biosynthesis of plasmalogen in mammalian cells has been reported to depend on aerobic conditions. Using liquid chromatography-tandem mass spectrometry, we found that the intracellular concentration of plasmalogen species containing a PUFA at the sn-2-position did not change for two days from the start of hypoxic culture in human colorectal cancer-derived Caco2 cells. At the third day of hypoxia, Caco2 cells showed the average increase rate of 2.6 times in ethanolamine plasmalogen and 2.9 times in choline plasmalogen depending on the molecular species compared with those in the second day of hypoxia. In normoxic culture, there was little quantitative change in any species of both ethanolamine and choline plasmalogens for three days. The up-regulations of mRNA of Ca2+-independent phospholipase A2β and cytoplasmic phospholipase A2γ as well as the down-regulation of lysoplasmalogenase observed in hypoxia were suggested to be responsible for the increase of plasmalogen in Caco2 cells under hypoxia.
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