NAD+激酶
氧化还原
酶
电子传输链
生物化学
化学
立体化学
有机化学
作者
Amna Mhamdi,Frank Van Breusegem,Graham Noctor
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 97-106
被引量:1
标识
DOI:10.1007/978-1-0716-2469-2_7
摘要
Pyridine nucleotides (NAD(H) and NADP(H)) are key redox carriers in cells and may also have other functions related to stress. These two molecules are crucial in linking metabolism to electron transport chains in photosynthesis and respiration, but they are also critical for ensuring redox signaling and homeostasis during episodes of stress. This is especially the case for NADPH, which must be generated from its oxidized form, NADP+, by key dehydrogenases. Here, we describe methods that can be used to assay contents and redox states of NAD(H) and NADP(H), as well as simple assays to measure the capacity of two key NADPH-generating enzymes.
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