化学
丙烯醛
叠氮化物
点击化学
共价键
碳化物
细胞器
癌症
光化学
组合化学
生物化学
催化作用
有机化学
铑
内科学
医学
作者
Ambara R. Pradipta,Hiroyuki Michiba,Anna Kubo,Motoko Fujii,Tomonori Tanei,Koji Morimoto,Kenzo Shimazu,Katsunori Tanaka
标识
DOI:10.1246/bcsj.20210387
摘要
Abstract Recently, we found that a bulky 2,6-diisopropylphenyl azide is more reactive than a simple phenyl azide toward acrolein. Herein, we conjugated tetramethylrhodamine (TAMRA) to the bulky phenyl azide and utilized it as a second-generation click-to-sense (CTS) probe to analyze cancer by targeting the cellular acrolein. Subsequently, we observed that this second-generation CTS probe could distinguish breast cancer tissues with higher sensitivity than the first-generation CTS probe. Moreover, adding Rh2(OAc)4 to the system promotes the formation of a metal carbenoid intermediate that accelerates the covalent attachment of the probe to an organelle in the cancer cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI