Comparative Study on Purification of IgG from Different Species by Protein “A” Affinity Chromatography

Monoclonal antibodies (MAbs) developed in 1975 by Georges Kohler have been applied in many areas of research. Staphylococcal protein-A binds to most mammalian IgG and can be employed for detecting or purifying such antibodies. Affinity chromatography on staphylococcal Protein A column is extensively used for purifying Monoclonal and Polyclonal antibodies. IgG from serum samples of different species were purified by the method described in Protein “A” Agarose Affinity Chromatography kit supplied by Bangalore Genie Pvt Ltd, Bangalore India. IgG from serum samples of different species eluted as a single major peak. The descending order of affinity of Protein “A” for IgG from serum of different species are pig ˃ human ˃ rabbit ˃ dog goat ˃ sheep ˃ buffalo ˃ chicken respectively.