脱氧核酶
化学
荧光团
催化作用
生物传感器
荧光
二价
水溶液中的金属离子
金属
检出限
核糖核酸
无机化学
纳米技术
组合化学
色谱法
生物化学
有机化学
物理
材料科学
基因
量子力学
作者
Runjhun Saran,Juewen Liu
标识
DOI:10.1021/acs.analchem.6b00327
摘要
Silver is a very common heavy metal, and its detection is of significant analytical importance. DNAzymes are DNA-based catalysts; they typically recruit divalent and trivalent metal ions for catalysis. Herein, we report a silver-specific RNA-cleaving DNAzyme named Ag10c obtained after six rounds of in vitro selection. Ag10c displays a catalytic rate of 0.41 min(-1) with 10 μM Ag(+) at pH 7.5 with 200 mM NaNO3, while its activity is completely inhibited with the same concentration of NaCl. Ag10c is highly specific for Ag(+) among all the tested metals. A catalytic beacon biosensor is designed by labeling a fluorophore and a quencher on the DNAzyme. Fluorescence enhancement is observed in the presence of Ag(+) with a detection limit of 24.9 nM Ag(+). The sensor shows a similar analytical performance in Lake Huron water. This is the first monovalent transition metal dependent RNA-cleaving DNAzyme. Apart from its biosensor application, this study strengthens the idea of exploring beyond the traditional understanding of multivalent ion dependent DNAzyme catalysis.
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