秆
纤维素
水解
酶水解
化学
木质素
生物量(生态学)
纤维素酶
傅里叶变换红外光谱
生物燃料
化学工程
有机化学
农学
生物技术
生物
园艺
工程类
作者
Shuang‐kui Du,Siqing Xia,Weiben Yang,Yanqin Wang,Meng Kuang,Lei Ma,Dan Fang,Dayun Zhou
标识
DOI:10.1016/j.carbpol.2015.12.056
摘要
Cotton stalk is a potential biomass for bioethanol production, while the conversion of direct saccharification or biotransformation of cotton stalk is extremely low due to the recalcitrant nature of lignocellulose. To enhance the enzymatic conversion of cotton stalks, the enzymatic saccharification parameters of high pressure assist-alkali pretreatment (HPAP) cotton stalk were optimized in the present study. Results indicated that a maximum reducing sugar yield of 54.7g/100g dry biomass cellulose was achieved at a substrate concentration of 2%, 100rpm agitation, 0.6g/g enzyme loading, 40°C hydrolysis temperature, 50h saccharification time, and pH 5.0. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy were used to identify structural changes in native, pretreated biomass and hydrolyzed residues. Structural analysis revealed large part of amorphous cellulose and partial crystalline cellulose in the HPAP cotton stalk were hydrolyzed during enzymatic treatment. HPAP cotton stalk can be used as a potential feed stock for bioethanol production.
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