Peltier device based thermoelectric generator is the environment friendly methodology than conventional cooling system. The main purpose to this system is cooling of different biomedical contents which are to be kept cold during travelling. Another purpose of this system is to design environment friendly cooling system. As in conventional cooling systems, there is lots of emission of gases which are harmful to environment as well as they harms ozone layer also. It leads to the severe problem like global warming. To overcome from this problem we designed a system which is friendly to the environment and which does not emits gases. Based on the theory given by scientists Thomas Seeback (1823) and Jean Peltier (1834) which are known as Seeback effect and peltier effect. We designed our system which is efficient and best alternative to the conventional cooling systems. When a DC current is passed through a Peltier device, the low temperature side absorbs heat and the high temperature side emits heat, so that a temperature difference exists across the sides of the peltier devices. A Peltier Module fitted between a aluminum cage and a heat extractor like an aluminum block for use as a cooling device is called a Cooler Unit. While using thermoelectric effect in system the efficiency of the system also increases. In this paper we give small details of thermoelectric generator. The model is tested and verified with proper hardware and software and results are found to be satisfactory.