Methods of primary solid tumor cell isolation and culture

Primary solid tumor cell culture is the initial culture set up directly from the solid tumors of donors. The tumor cells generated from primary cell culture are the closest specimen to the original tissues and can truly reflect the characteristics of tumor cell growth in vivo. Using primary solid tumor cell cultures, researchers have obtained more persuasive data in tumor studies. This review discusses the methods of primary solid tumor cell isolation and culture, factors restricting its extensive applications and the keys to solving the associated problems. Key words: Solid tumor; Primary cell; Isolation and culture