DNA Origami Nanostructures with Scaffolds Obtained from Rolling Circle Amplification

As a guiding backbone of origami structural folding, the long single-stranded scaffold is the key enabler of all kinds of complex DNA nanostructures. However, the folding of most origami nanostructures depends heavily on very limited species of scaffolds. It is desirable to have a wider choice of scaffolds for the extended design space. Here we develop a new method based on rolling circle amplification to prepare scaffolds of custom lengths and sequences. We demonstrate that amplicons templated by viral genome and plasmid can serve as scaffolds to fold DNA origami nanostructures.