小眼畸形相关转录因子
酪氨酸酶
红景天
奶油
黑色素
生物化学
水解物
化学
磷酸化
蛋白激酶A
红景天
蛋白激酶B
药理学
生物
转录因子
酶
红景天苷
基因
水解
作者
Hsiu‐Mei Chiang,Yin Chih Chien,Chieh Hsi Wu,Yueh Hsiung Kuo,Wen-Lan Wu,Yu Yun Pan,Yu Han Su,K.-C. Wen
标识
DOI:10.1016/j.fct.2013.12.032
摘要
We investigated the effects of an aqueous alcohol extract of Rhodiola rosea (R. rosea) and its hydrolysate on melanin synthesis and the mechanisms mediating the activity. The ratio of tyrosol to salidroside was 2.3 in hydroalcoholic extract, and 51.0 in hydrolysate. We found that R. rosea extract and its hydrolysate inhibited melanin synthesis and tyrosinase activity in mouse melanoma cells (B16F0 cells). R. rosea extract also inhibited gene and protein expression of melanocortin 1 receptor (MC1R) and inhibited c-AMP response element binding protein (CREB) phosphorylation, suppressed the activation of AKT and glycogen synthase kinase-3 beta (GSK3β), and inhibited the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase-related protein 1 (TRP-1). R. rosea hydrolysate inhibited the phosphorylation of CREB, the activation of AKT and GSK3β, and the expression of MITF and tyrosinase. Our results suggest that R. rosea extract is a novel tyrosinase inhibitor and that it exerts its effects by regulating the CREB/MITF/tyrosinase pathway in B16F0. Further in vivo studies are needed to determine the effectiveness of R. rosea extract as a skin whitening agent.
科研通智能强力驱动
Strongly Powered by AbleSci AI