Expressions of P16~(INK4A) and Ki-67 and HPV infection in cervical intraepithelial neoplasia and cervical cancer

Objective:To explore the expressions of P16INK4A and Ki-67 in cervical intraepithelial neoplasia(CIN) and cervical cancer and their relationships with HPV16/18 infection.Methods:94 paraffin-embedded cervical samples were selected,including 20 samples of chronic cervicitis,13 samples of CIN Ⅰ,17 samples of CIN Ⅱ,20 samples of CIN Ⅲ and 24 samples of cervical cancer,immunohistochemical method was used to detect the expression levels of P16INK4A and Ki-67,and PCR technology was used to detect HPV16/18 DNA.Results:The positive expression rates of P16INK4A in chronic cervicitis group,CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group and cervical cancer group were 0.0%,53.8%,76.5%,95.0% and 100.0%,respectively,there was significant difference in positive expression rate of P16INK4A between CIN Ⅲ group,cervical cancer group and the other groups(P0.05);the positive expression rates of Ki-67 in chronic cervicitis group,CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group and cervical cancer group were 0.0%,69.2%,88.2%,95.0% and 91.7%,respectively,there was significant difference in positive expression rate of Ki-67 between CIN Ⅲ group,cervical cancer group and chronic cervicitis group;HPV16/18 mainly infected the patients with CIN of high grade and cervical cancer;the expression levels of P16INK4A and Ki-67 were correlated positively with the degree of cervical lesions and HPV16/18 infection.Conclusion:HPV16/18,P16INK4A and Ki-67 are related to the occurrence and development of cervical cancer.