Application of matrine in mice after sciatic nerve injury in spinal motor neurons

Objective To investigate the BALB / c mice after sciatic nerve injury in the sciatic nerve after application of matrine corresponding spinal segment in the expression of gap-43.Methods Healthy adult BALB / c mice 320,consistent with unilateral sciatic nerve cut,the randomized dose,at different times for L4-6 spinal segment,using Real time-PCR detection of the corresponding spinal segment of sciatic nerve gap-43 expression changes,while the LFB myelin staining of sciatic nerve recovery.Results Realtime-PCR results indicate that high-dose and medium-dose group 12h,24h 3d 5d 1w,2w,4w,gap-43 expression was significantly higher than low-dose group and blank control group,8w no significant difference between the groups.LFB staining showed that the recovery of the sciatic nerve high-dose group was significantly better than low-dose group and control group.Conclusion application of sciatic nerve injury in the spinal cord of matrine on the corresponding segments of sciatic nerve cells in the activation of gap-43 play a catalytic role in reducing the infiltration of inflammatory cells to achieve the purpose of promoting nerve regeneration.