The contents of fructose, glucose, sucrose and total sugar of watermelon fruits with eight groups of different ploidy including diploid (2x), triploid (3x) and tetraploid (4x) were measured. The results showed that the fructose, glucose, sucrose and total sugar contents were divergent in different ploidy watermelon as follows: total sugar and fructose maked the same trend, 4x3x2x; glucose, 4x2x3x; sucrose, 3x4x2x.