High-performance liquid chromatography method for the analysis of sodium benzoate

Sodium Benzoate use as preservative in pharmaceutical and food industry. Only titrimetric methods are available in pharmacopeias for the determination of sodium benzoate assay. Chromatography & stability indicating method is not available for the determination of sodium benzoate assay. Rapid economical, reproducible and simple direct method was developed and validated for determination of sodium benzoate using high performance liquid chromatography (HPLC). Sodium Benzoate concentration estimated with isocratic elution using a mixture of buffer and methanol and water (60: 40 v/v) within 10 min, Flow rate 1.00ml, and wavelength 254nm and column C18. This method was suitable and validated for specificity, linearity, precision, accurate and ruggedness. The proposed method was successfully applied in the determinate of raw material assay for sodium benzoate.