英哈
胚泡
男科
胚胎
化学
内分泌学
人类受精
生育率
生物
体外成熟
胚胎发生
卵巢
内科学
医学
解剖
遗传学
人口
病理
异烟肼
肺结核
环境卫生
作者
Lun Zhang,Xin Liang,Xiaohui Jiang
出处
期刊:PubMed
日期:2012-05-01
卷期号:43 (3): 348-51
摘要
To study the effects of activin A (ACTA) and inhibin A (INHA) on the in vitro maturation (IVM) of mice immature eggs and early embryonic development.1. The mice oocytes were cultured in the culture medium contained different concentration of ACTA and INHA (50 ng/mL, 100 ng/mL, 200 ng/mL) to find the optimum concentration; 2. The effects of different concentration of ACTA, INHA, ACTA+ INHA on the mature rates of mice oocytes were compared; 3. The effects of different concentration of ACTA, INHA, ACTA+INHA on the fertility rates and blastocysts formation rates of IVM mice eggs were also compared.The mature rates of mice oocytes in culture medium contained (100 ng/mL and 200 ng/mL) ACTA and INHA were significantly higher than that contained 50 ng/mL ACTA and INHA (P < 0.05); There was no difference between 100 ng/mL and 200 ng/mL groups. The mature rates, fertility rates and blastocyst rates of mice oocytes were significantly higher than those of control (P < 0.05).The mature rates, fertility rates and embryonic development potential after fertilization of mice oocytes were significantly promoted by ACTA, INHA. The optimum concentration of ACTA and INHA in culture medium was 100 ng/mL.
科研通智能强力驱动
Strongly Powered by AbleSci AI