[Objective]The purpose was to extract polysaccharide in Cynanchum auriculatum Aerial parts and determine content of polysaccharide.[Method] The prepration,purification and determination of polysaccharides in Cynanchum auriculatum Aerial parts by microwave technique were investigated.The optimal technics parameter of extracting polysaccharides from Cynanchum auriculatum Aerial parts were determined through orthogonal test.Then this technique was used the extraction polysaccharides in Cynanchum auriculatum Aerial parts whose content were assayed by the phenol-sulfuric acid method.[Result]The results showed that the optimal technical parameters were liquid-solid ratio 1∶25 and the extracting time 80 s under 80% microwave power.The concentration of polysaccharide in Cynanchum auriculatum Aerial parts was 2.57%.[Conclusion]This research will provide theoretical evidence for utilization of Cynanchum auriculatum Aerial parts.