Expression of the hBD-2 and change of NF-κB activity in human primary epitheliums induced by LPS

AIM:To investigate the expression of human β-defensin-2(hBD-2)mRNA and the changes of NF-κB activity induced by lipopolysaccharide(LPS)in human primary epitheliums.METHODS:The pulmonary primary epitheliums from human were stimulated with LPS and the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR).The nuclear factor-kappa B(NF-κB)binding activity was analyzed by electrophoretic mobility shift assays.RESULTS:The hBD-2 mRNA was detected 2 hours after LPS stimulation and the expression of hBD-2 showed a dose-dependent manner.The NF-κB was activated remarkably half an hour after LPS stimulation.The supershifts assays indicated that the p65-p50 heterodimer formed complexes of NF-κB,that was involved in the activated of NF-κB.CONCLUSION:LPS induces the expression of hBD-2 mRNA in a dose-dependent manner.The p65-p50 heterodimer-formed complexes of NF-κB plays an important role in the regulation of hBD-2 gene expression in response to LPS.