Determination of hesperidin and naringinin in citrus peel by RP- HPLC simultaneously

An RP-HPLC method was established for the determination of hesperidin and naringin in the citrus peel under different extracted conditions,and at the same time UV spectrum of two components was detected by means of Photodiode Array Detector(PAD).Hesperidin and naringin were separated on an ODS C18 column(150mm×4.6mm,5μm)with methanol-water(volume ratio of 40/60)as the mobile phase and detected at 270nm.The flow rate was 1.0mL/min.The column temperature was set at 25℃.The calibration curves were linear in the ranges of 0.007168~0.28g/L and 0.012288~0.48g/L for hesperidin and naringin respectively.Determination of hesperidin was 17.5~21.9mg/g(RSD=0.77%~1.86%)and naringin determination was 2.14~2.78mg/g(RSD=0.98%~1.59%).The average recoveries were 98.5%~99.4%(RSD=0.69%~1.78%)for hesperidin and 99.3%~101.1%(RSD=0.43%~1.45%)for naringin,and the detection limits were 0.28μg and 0.16μg respectively.This method was simple,accurate,reproducible and had wider linear range,which could be adapted to evaluate the quality of plant that contains two component.