表面等离子共振
肽
抗体
亲和层析
生物传感器
类风湿性关节炎
化学
特异性抗体
分子生物学
生物化学
免疫学
医学
材料科学
纳米技术
生物
酶
纳米颗粒
作者
Feliciana Real-Fernández,Giada Rossi,Filomena Panza,Federico Pratesi,Paola Migliorini,Paolo Rovero
出处
期刊:Springer eBooks
[Springer Nature]
日期:2015-01-01
卷期号:: 267-274
被引量:5
标识
DOI:10.1007/978-1-4939-2999-3_23
摘要
Surface plasmon resonance (SPR) technique is extremely interesting in immunology because it has the potential to directly visualize biomolecular interactions in real-time monitoring antibody affinity, one of the parameters affecting pathogenicity in autoimmune diseases. Herein we describe the affinity evaluation of anti-citrullinated peptide antibodies (ACPA) to a peptide-based biosensor by SPR. The method describes the purification of ACPA isolated from rheumatoid arthritis (RA) patients using affinity columns, the strategy employed for the immobilization of citrullinated peptides onto a sensor chip, and the evaluation of the specific binding of purified ACPA to immobilized peptides.
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