[Objective]The aim was to study the action mode of high mobility group B(HMGB)proteins of higher eukaryotic cells in the transcriptional regulation of plant stress responses.[Method]The At2G33450 gene encoding AT2G34450 protein in Arabidopsis thaliana was cloned.Then,binary vectors carrying the gene were transformed into Arabidopsis thaliana and the over-expression strains were selected to detect the influences of environmental stimuli on transgenic Arabidopsis.[Result]Under salt or drought stress,the transgenic Arabidopsis plants which over-expressed At2G33450 displayed retarded germination and subsequent growth compared with wild-type plants.[Conclusion]At2G33450 protein of HMGB protein family played an important role in the growth and development of Arabidopsis plants under various stress conditions.