Optimization of the reactive conditions for ATP production from adenine

The reactive conditions for ATP production from adenine by Mucor were investigated by single factor experiment.A new reaction liquid using Na2HPO4 instead of K2HPO4(which is more expensive) was proposed and investigated.Based on shaking flask experiments with orthogonal experiment,the optimum reaction liquid was obtained.The effects of stage addition of Na2HPO4 on ATP yield and reaction time were investigated.As a result,the optimum pH was 6.5,and the ATP yield was increased remarkably by using 1% xylene at the reaction time of 2 h and placing the reaction liquid staticly for 2 h after the reaction time of 2 h.The optimum reaction liquid was Mucor 500 g/L,glucose 120 g/L,Na2HPO4 90 g/L,MgCl2 4 g/L,MnCl2 2 g/L,adenine 3 g/L,xylene 1%.The ATP yield was increased from 6.218 g/L to 10.046 g/L under the condition of stage addition of Na2HPO4.The yield was increased by 61.56% and the reaction time was decreased by 2 hours.