Determination of 11 β_2-agonists in Foodstuff of Animal Origin by Liquid Chromatograph with Tandem Mass Spectrometric Detection

The method for determination of β2-agonists (salbutamol, terbutaline, cimaterol, cimbuterol, ractompamine, clenbuterol, bromobuterol, isoxsuprine, mabuterol, mapenterol, bromclenbuterol) residues in liver and meat of pig was developed by HPLC/MS-MS. The sample was extracted in acetate buffer (pH 5.2), hydrolyzed by β-glucuronidase-aryl sulfatase. The protein was precipitated with perchloric acid. The extraction passed through a cation exchange column (MCX 3 mL, 60 mg), Clenbuterol-D9 Salbutamol-D3 were used as an internal standard, then eleven β2-agonists can be determined at one time by HPLC/MS-MS. The experimental results from spiked liver sample indicate that the recoveries range from 89.4%-110.5% and the RSD is 1.1%-2.8%. The limits of detection (LODs) is 0.25 μg·kg-1. The method is suitable for food safety.