暗室
荧光
荧光团
荧光显微镜
显微镜
生命科学中的荧光
显微镜
光学
吸收(声学)
分子
材料科学
波长
光学显微镜
化学
光电子学
物理
扫描电子显微镜
有机化学
标识
DOI:10.1002/0471142735.im2102s48
摘要
Abstract Absorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular structures. This unit discusses the optics and other components of the fluorescence microscope, their functions and adjustments, and the digital darkroom.
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