微分脉冲伏安法
生物传感器
胶体金
循环伏安法
电化学气体传感器
分析物
玻璃碳
分析化学(期刊)
拉曼光谱
化学工程
化学
碳纤维
作者
Danfeng Sun,Hongji Li,Mingji Li,Cuiping Li,Lirong Qian,Baohe Yang
标识
DOI:10.1016/j.bios.2019.02.045
摘要
Abstract We proposed label-free and sandwich-type electrochemical immunosensors for the quantification of alpha-fetoprotein (AFP), which plays a critical role in the early diagnosis of primary liver cancer. Both detection strategies involved monitoring of the electrochemical response current of the AuPt-vertical graphene (VG)/glassy carbon electrode (GCE) for the oxidation of the redox probe, methyl orange. AuPt nanoparticles, which have good biocompatibility and excellent conductivity, were used to immobilize the primary antibody, Ab1, while VG sheets helped accelerate electron transfer at the liquid-solid and solid-solid interfaces. The sensing electrode played a key role in signal amplification, and the sensitivity of the sandwich-type sensor was higher than that of the label-free sensor. The former exhibited a wide linear range from 1 fg mL−1 to 100 ng mL−1 and a low detection limit of 0.7 fg mL−1 under the optimal experimental conditions. The excellent performance of the AuPt-VG/GCE-based immunosensors in AFP detection in human serum, coupled with their good stability, makes them promising tools for the clinical monitoring of AFP and other tumor markers.
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