Thawingan organoid cryovial v1

This SOP defines the procedure for thawing a frozen cryovial of organoids into 1 well of a 6 well plate for further culture. It has been developed by the organoid derivation team within the Cellular Generation and Phenotyping Group at the Wellcome Sanger Institute. The team has extensive experience passaging and expanding organoid models. The method described has mainly been used for cancer organoids with successful propagation of organoids derived from colon, pancreas and oesophageal tumours.