A ratiometric fluorescent probe for the detection of hypochlorous acid in living cells and zebra fish with a long wavelength emission

Abstract A ratiometric fluorescence probe, NClO, for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative. Probe NClO displayed a red emission (λmax = 615 nm). In the presence of HClO, the solution of probe NClO gave off a strong green fluorescence (λem, max = 520 nm) with a rapid response (within seconds). This probe had been applied to image HClO in living cells and zebra fish.