材料科学
光子上转换
光动力疗法
瓜尔胶
体外
共轭体系
结直肠癌
药物输送
介孔二氧化硅
光热治疗
癌症研究
纳米技术
生物物理学
癌症
化学
生物化学
聚合物
医学
介孔材料
有机化学
光电子学
生物
发光
催化作用
复合材料
内科学
作者
Balmiki Kumar,Aparna Murali,A B Bharath,Supratim Giri
出处
期刊:Nanotechnology
[IOP Publishing]
日期:2019-05-23
卷期号:30 (31): 315102-315102
被引量:33
标识
DOI:10.1088/1361-6528/ab116e
摘要
Multimodal therapeutic approach towards colorectal cancer (CRC) holds great promise. There is, however, no convincing strategy reported to date that employs a multimodal strategy in CRC treatment. The present study reports an intense green-emitting core–shell photoluminescent upconversion (CSGU) nanocrystal engineered to synergistically perform photodynamic and enzyme-triggered delivery of the chemotherapeutic agent for an enhanced therapeutic outcome on HT-29 colon carcinoma cells in vitro. The photodynamic activity is achieved by the energy transfer between CSGU and the chemically conjugated Rose Bengal (RB) molecules that are further protected by a mesoporous silica (MS) layer. The chemical assay demonstrates a remarkable FRET mediated generation of 1O2 under NIR (980 nm) excitation. The outermost MS layer of the nanoplatform is utilized for the loading of the 5FU anticancer drug, which is further capped with a guar gum (GG) polysaccharide polymer. The release of the 5FU is specifically triggered by the degradation of the GG cap by specific enzymes secreted from colonic microflora, which otherwise showed 'zero-release behavior' in the absence of any enzymatic trigger in various simulated gastro-intestinal (GI) conditions. Furthermore, the enhanced therapeutic efficacy of the nanoplatform (CSGUR-MSGG/5FU) was evaluated through in vitro studies using HT-29 CRC cell lines by various biochemical and microscopic assays by the simultaneous triggering effect of colonic enzyme and 980 nm laser excitation. In addition, the strong visible emission from the nanoplatform has been utilized for NIR-induced cellular bioimaging.
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