免疫原性
化学
结合
糖复合物
佐剂
半抗原
结合疫苗
抗原
连接器
表位
效价
抗体
生物化学
分子生物学
免疫学
生物
操作系统
数学分析
计算机科学
数学
作者
Xin Meng,Chuanming Ji,Chao Su,Di Shen,Yaxin Li,Peijie Dong,Yuan Ding,Mujin Yang,Shao‐Tao Bai,Demei Meng,Zhen‐Chuan Fan,Yang Yang,Peng Yu,Tao Zhu
标识
DOI:10.1016/j.ejmech.2017.03.058
摘要
A PG-tb1 hapten from the West Beijing strains of Mycobacterium tuberculosis cell wall has been efficiently synthesized and conjugated to CRM197 in a simple way as linker-equipped carbohydrate by applying squaric acid chemistry for an original neoglycoprotein, creating a potent T-dependent conjugate vaccine. The intermediate monoester can be easily purified and the degree of incorporation can be monitored by MALDI-TOF mass spectrometry. After administered systemically in mice without any adjuvant, the conjugate induced high antigen-specific IgG levels in serum. Furthermore, following the third immunization, significant antibody titers frequently exceeding 0.8 million were observed in the sera of mice vaccinated with PG–CRM197 conjugate which showed the potential for preparation of TB vaccine.
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