化学
分子印迹聚合物
沉淀聚合
甲基丙烯酸
弗伦德利希方程
水溶液
吸附
聚合
朗缪尔吸附模型
分子印迹
核化学
高分子化学
聚合物
有机化学
自由基聚合
选择性
催化作用
作者
Yao Xiao,Rong Xiao,Jian Tang,Qiankun Zhu,Xiaoming Li,Yan Xiong,Xuewen Wu
出处
期刊:Talanta
[Elsevier]
日期:2017-01-01
卷期号:162: 415-422
被引量:55
标识
DOI:10.1016/j.talanta.2016.10.014
摘要
The molecularly imprinted polymers (MIP) were prepared via aqueous RAFT precipitation polymerization, with aristolochic acid I (AAI) as the template molecule, AA as the functional monomer, EGDMA as the cross-linker, AIBN as the chain initiator, CTP as the chain transfer agent and 80% (g/g) DMF-aqueous solution as the porogen. The differential UV–vis spectra revealed that a cooperative hydrogen-bonding complex between AAI and AA might be formed at the molar ratio of 1:3 in prepolymerized system. The synthesized MIPs were characterized by FTIR spectra, solid UV–visible absorption spectra, nitrogen adsorption-desorption isotherms and scanning electron microscope, which proved that the MIPs and NIPs have the similar chemical structures and the adding of AAI could affect the size and morphology of the microspheres. UV–visible absorption spectra and High-performance liquid chromatography (HPLC) was used to investigate the adsorption and recognition properties of the MIPs. The Scatchard isotherm model described that the binding sites independently acted. The Langmuir isotherm model suggested an excellent imprinting effect owing to the presence of a large number of specific binding sites on the MIP. The Freundlich model indicated that the AAI could be readily absorbed by MIP. Selective absorption of the template molecule was demonstrated in presence of its analogous compounds, benzoic acid and nitrobenzene. The recycling experiments implied that the MIP could be reused to further selective recognition and separation to AAI for six times at least. MIP was developed for removal of AAI from the Aristolochia manshuriensis extraction. The results indicated that 25 mg of MIP could remove the AAI below the HPLC detection limits (6.47 ng mL-1) from 5.0 mL of the extraction (CAAI=0.0018 mg mL-1) with the recovery of AAI to 91.50% (n=3, SD=4.24%). Therefore, it is clearly revealed that the MIP can be a useful tool to remove toxic compounds from natural products.
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