小胶质细胞
脂多糖
肿瘤坏死因子α
体外
细胞生物学
程序性细胞死亡
细胞培养
神经元
运动神经元
生物
分泌物
细胞
分子生物学
化学
免疫学
炎症
神经科学
细胞凋亡
生物化学
遗传学
脊髓
作者
Bei He,Weiyan Wen,Michael J. Strong
标识
DOI:10.1016/s0165-5728(02)00141-8
摘要
We have studied the interactions between activated microglia and injured motor neurons using an immortalized murine microglial cell line (BV-2) stimulated with either lipopolysaccharide (LPS) (Escherichia coli) or supernatant from serum-deprived motor neurons (NSC-34 cell line). Both stimuli induced BV-2 activation. Although both BV-2 supernatants induced a subsequent increase in NO generation in otherwise healthy NSC-34 cells, only LPS-activated microglial supernatant induced NSC-34 cell death through a TNF-alpha-dependent pathway. However, we observed a 20-fold increase in the amount of TNF-alpha required to kill NSC-34 cells in the absence of LPS-activated BV-2 cell supernatant, indicating that microglia secrete factor(s) that facilitate TNF-alpha-mediated motor neuron death in vitro.
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