耐寒性
突变体
生物
转录因子
普通小麦
冷应激
抄写(语言学)
非生物成分
细胞生物学
基因
遗传学
冷敏
冬小麦
非生物胁迫
基因表达
DNA结合蛋白
小干扰RNA
核糖核酸
分子生物学
植物
作者
Liu Jinyuan,Yaoyao Jiang,Lei Zhao,Qianhui Xi,Awen He,Fangyu Xiang,Jiaqi Li,Lingran Zhang,Congwei Sun,Ning Zhang,Jie Yang,F. Chen
摘要
Abstract Low temperature as one of the most important abiotic stresses has caused serious wheat production loss worldwide. We identified TaWRKY115 affecting cold tolerance in wheat by integration of genome-wide association study and RNA sequencing. Overexpression and CRISPR/Cas9-mediated gene-editing revealed TaWRKY115 positively modulating cold tolerance in wheat. Transcription factor TaSP1 negatively regulates wheat cold tolerance by binding to the promoter of TaWRKY115 in cold-sensitive haplotype. TaWRKY115 inhibited TaMYB4 at both protein and transcription levels. BSMV-mediated silenced wheat plants and EMS mutants of TaMYB4 possessed increased wheat cold tolerance. We further revealed that TaMYB4 repressed expressions of the TaCBF family through binding to their promoters. BSMV-mediated silenced wheat plants and EMS mutants of TaCBF12d exhibited decreased cold tolerance in wheat. This study brought forth a new model TaSP1-TaWRKY115-TaMYB4, regulating wheat cold tolerance through the TaCBF pathway and provided valuable cold tolerance genes for wheat breeding programs.
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