Expression and Functional Role of Cdx2 in Intestinal Metaplasia of Cystitis Glandularis

No AccessJournal of UrologyInvestigative Urology1 Sep 2013Expression and Functional Role of Cdx2 in Intestinal Metaplasia of Cystitis Glandularis Ming-en Lin, Danying Huang, Bi-hua Deng, Yi-song Lv, Lu Rong, and You-sheng Yao Ming-en LinMing-en Lin Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China Department of Urology, First Affiliated Hospital of Medical College Shantou University, Shantou City, Guangdong, People's Republic of China More articles by this author , Danying HuangDanying Huang Department of Urology, Center of Experiment and Training, Vocational and Technical College of Jieyang, Jieyang City, Guangdong, People's Republic of China More articles by this author , Bi-hua DengBi-hua Deng Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author , Yi-song LvYi-song Lv Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author , Lu RongLu Rong Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author , and You-sheng YaoYou-sheng Yao Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.03.109AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Cdx2 is an essential transcription factor in intestinal epithelial cell differentiation and proliferation. However, to our knowledge the expression and role of Cdx2 in the development of intestinal cystitis glandularis, a metaplastic lesion induced by chronic inflammation, remained to be explored. Materials and Methods: Real-time polymerase chain reaction was used to examine Cdx2, LI-cadherin and villin expression in typical and intestinal cystitis glandularis, and normal bladder tissue. Cdx2 cDNA was subcloned to the retroviral vector pLNCX2 for subsequent transfection into human bladder urothelium cells and rat bladder urothelium. Cdx2 mRNA and protein levels, and cell morphology and proliferation were assessed after transfection using real-time polymerase chain reaction, phase contrast microscopy, transmission electron microscopy and MTT assay, respectively. Results: Higher mRNA levels of Cdx2, villin and LI-cadherin were detected in intestinal cystitis glandularis compared to normal bladder and typical cystitis glandularis. Only Cdx2 groups attained statistical significance (p <0.001). Retroviral over expression of Cdx2 resulted in increased mRNA and protein expression of Cdx2 as well as villin and LI-cadherin levels, and increased cell proliferation. A distinct change in cellular morphology, in which cells resembled intestinal-like cells, was also observed in vitro and in vivo. Conclusions: Cdx2 may have a critical role in regulating intestinal metaplasia in cystitis glandularis. Further studies are planned to assess the potential of using Cdx2 as a marker and therapeutic target for cystitis glandularis. References 1 : Compare the curative effect of cystitis glandularis between transurethral resection and transurethral resection and followed bladder instillation with Thio-TEPA. Chin J Surg2002; 40: 239. Google Scholar 2 : Divergent pathway of intestinal metaplasia and cystitis glandularis of the urinary bladder. Mod Pathol2006; 19: 1395. Google Scholar 3 : Cystitis cystica glandularis masquerading as a bladder tumor. Int Urol Nephrol2001; 33: 635. 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Google Scholar © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 190Issue 3September 2013Page: 1083-1089 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.Keywordsurinary bladdercystitisCDX2 protein, humanvillinCDH17 protein, humanMetrics Author Information Ming-en Lin Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China Department of Urology, First Affiliated Hospital of Medical College Shantou University, Shantou City, Guangdong, People's Republic of China More articles by this author Danying Huang Department of Urology, Center of Experiment and Training, Vocational and Technical College of Jieyang, Jieyang City, Guangdong, People's Republic of China More articles by this author Bi-hua Deng Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author Yi-song Lv Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author Lu Rong Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author You-sheng Yao Department of Urology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangdong, People's Republic of China More articles by this author Expand All Advertisement PDF downloadLoading ...