Stereoselective Hydroxylation of (+)-dihydroperfamine Cunninghamella echinulata

The alkaloid (+)-dihydroperfamine (1) was stereoselectively hydroxylated in 47% yield at the 5" position, using the fungus Cunninghamella echinulata. The structure and stereochemistry of metabolite 2 have been confirmed by 1D and 2D NMR spectroscopy, including a NOESY experiment. The hydroxylation pattern of 2 is similar to that found in the alkaloid haplatine (3).