生物
衣壳
重组DNA
核定位序列
九氟化硫
NLS公司
亚细胞定位
分子生物学
聚腺苷酸
融合蛋白
核运输
DNA
圆环病毒
病毒学
猪圆环病毒
基因
细胞生物学
信使核糖核酸
遗传学
病毒
细胞核
夜蛾
作者
Xiang Qiu,Jinfeng Zou,Xin Wang,Yani Sun,Jiming Gao,Zhixun Xie,Yu Wang,Yanli Zhu,Shijin Jiang
出处
期刊:Virology
[Elsevier]
日期:2013-02-01
卷期号:436 (1): 112-117
被引量:12
标识
DOI:10.1016/j.virol.2012.10.035
摘要
The capsid protein (CP) of duck circovirus (DuCV) is the major immunogenic protein and has a high proportion of arginine residues concentrated at the N terminus of the protein, which inhibits efficient mRNA translation in prokaryotic expression systems. In this study, we investigated the subcellular distribution of DuCV CP expressed via recombinant baculoviruses in Sf9 cells and the DNA binding activities of the truncated recombinant DuCV CPs. The results showed that two independent bipartite nuclear localization signals (NLSs) situated at N-terminal 1-17 and 18-36 amino acid residue of the CP. Moreover, two expression level regulatory signals (ELRSs) and two DNA binding signals (DBSs) were also mapped to the N terminus of the protein and overlapped with the two NLSs. The ability of CP to bind DNA, coupled with the karyophilic nature of this protein, strongly suggests that it may be responsible for nuclear targeting of the viral genome.
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