This experiment was conducted to investigate the effect of dietary supplementation of the various materials contained lutein on the lutein accumulation in egg yolk. The laying hens were fed corn-soybean meal based diets containing 0, 0.6, 1, 3, 5 or 10 ppm of lutein. The lutein content of egg yolk was linear increased by increasing the supplementation level of lutein. The spinach 10 treatment, lutein content was the highest than that those treatments from egg yolk. But the efficiency of lutein accumulation in egg yolk was significantly higher in lutein extract treatment as compared to that of raw materials treated groups. The results of this experiment suggested that the dietary supplementation of lutein extract was more effective than that of raw materials for producing the lutein enriched eggs.