细胞生物学
生物
免疫学
启动(农业)
细胞分化
抗体
白细胞介素21
免疫系统
细胞生长
离体
抗原
CD40
B细胞
T细胞
细胞毒性T细胞
体内
体外
基因
发芽
生物技术
植物
生物化学
遗传学
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 111-119
被引量:4
标识
DOI:10.1007/978-1-4939-7474-0_8
摘要
Upon priming by antigens, B cells undergo activation, proliferation, and differentiation into antibody-secreting cells. During thymus-dependent (TD) antibody responses, the proliferation and differentiation of antigen-primed B cells essentially rely on the helper function from CD4+ T cells. Follicular helper T (Tfh) cells constitute a specialized Th subset that localizes in close proximity to B cells and supports B cell proliferation and differentiation through co-stimulatory receptors and cytokines. Impaired Tfh-mediated B cell proliferation and differentiation were observed in patients with immunodeficiency, while overactivation of this process may lead to dysregulated immune responses seen in autoimmune disorders. Here, we describe an ex vivo coculture assay using circulating Tfh cells and B cells isolated from human blood. This method can be used to examine the function of patients' B cells for proliferation, differentiation, and antibody secretion, mediated by the physiological help from Tfh cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI