阴沟肠杆菌
最小抑制浓度
微生物学
细胞内
化学
流出
溶解
抗菌活性
菌丝体
膜透性
最低杀菌浓度
膜
多糖
细菌
核化学
肠杆菌科
抗生素
生物化学
生物
大肠杆菌
植物
基因
遗传学
作者
Jun Liu,Zhujin Xu,Zhen Guo,Zuguo Zhao,Yi Zhao,Xin Wang
标识
DOI:10.1016/j.carbpol.2018.04.114
摘要
In this study, a polysaccharide (ECP) was isolated from the mycelium of Enterobacter cloacae and was found to exhibit strong antibacterial activities against E. cloacae producing SHV-12 ESBL with the increase of the inhibition zone diameter. Its minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were 12.5 mg/L and 25 mg/L, respectively. ECP at these concentrations immediately inhibited planktonic growth of the bacteria especially at the time from 2 to 10 h. Flow cytometry analysis further revealed that almost all the bacterial cells were damaged following ECP treatment. The permeability of the cytoplasmic membrane of E. cloacae was increased when ECP concentrations increasing, as evidenced by an influx of Na and an efflux of K, P or S, the leakage of intracellular ATP and the UV-absorbing substances, as well as the depolarization of the cytoplasmic membrane, indicating that bactericidal activity of ECP was achieved by inducing cell membrane damage.
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