二芳基乙烯
光致变色
拉曼光谱
材料科学
显微镜
拉曼散射
光化学
拉曼显微镜
荧光
荧光寿命成像显微镜
光电子学
光学
化学
纳米技术
物理
作者
Jianpeng Ao,Xiaofeng Fang,Xianchong Miao,Jiwei Ling,Hyung Seok Kang,Sungnam Park,Changfeng Wu,Minbiao Ji
标识
DOI:10.1038/s41467-021-23407-2
摘要
Abstract Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding “on” or “off” SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.
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