诱导多能干细胞
生物
体细胞
体细胞核移植
再生医学
细胞生物学
重编程
干细胞
外胚层
胚胎干细胞
生物技术
基因
遗传学
原肠化
胚泡
胚胎
胚胎发生
作者
Qianqian Zhu,Fengchong Wang,Dengfeng Gao,Jie Gao,Guilin Li,Deling Jiao,Gaoxiang Zhu,Kaixiang Xu,Jianxiong Guo,Tianzhi Chen,Suying Cao,Minglei Zhi,Jinying Zhang,Yingjie Wang,Xiaowei Zhang,Danru Zhang,Yixuan Yao,Jinlin Song,Hong‐Jiang Wei,Jianyong Han
摘要
Genome integration-free pig induced pluripotent stem cells (iPSCs) bring tremendous value in pre-clinical testing of regenerative medicine, as well as conservation and exploitation of endangered or rare local pig idioplasmatic resources. However, due to a lack of appropriate culture medium, efficient induction and stable maintenance of pig iPSCs with practical value remains challenging. Here, we established an efficient induction system for exogenous gene-independent iPSCs under chemically defined culture condition previously used for generation of stable pig pre-gastrulation epiblast stem cells (pgEpiSCs). WNT suppression was found to play an essential role in establishment of exogenous gene-independent iPSCs. Strikingly, stable integration-free pig iPSCs could be established from pig somatic cells using episomal vectors in this culture condition. The iPSCs had pluripotency features and transcriptome characteristics approximating pgEpiSCs. More importantly, this induction system may be used to generate integration-free iPSCs from elderly disabled rare local pig somatic cells and the iPSCs could be gene-edited and used as donor cells for nuclear transfer. Our results provide novel insights into potential applications for genetic breeding of livestock species and pre-clinical evaluation of regenerative medicine.
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