Selective Determination of Norfloxacin in Pharmaceutical Formulations and Human Urine Samples Using Poly(8-aminonaphthaline-2-sulfonic Acid)-Modified Glassy Carbon Electrodes

In this study, a glassy carbon electrode was modified potentiodynamically with poly(8-aminonaphthaline-2-sulfonic acid) [poly(ANSA)/GCE] for the detection of norfloxacin (NFN) in tablet formulations and human urine samples. Improvement of the effective surface area of the modified electrode and decreased charge-transfer resistance confirmed surface modification of the GCE by a conductive poly(ANSA) film. The appearance of an oxidative peak without a reductive peak in the reverse scan direction showed the irreversibility of the electrochemical oxidation of NFN in both the bare GCE and poly(ANSA)/GCE. A better coefficient of determination for the peak current on the square root of the scan rate (R² = 0.99514) than the scan rate (R² = 0.97109), indicating the oxidation of NFN at the poly(ANSA)/GCE, was predominantly diffusion mass transport-controlled. Under optimized pH and square wave parameters, the voltammetric current response of NFN at the poly(ANSA)/GCE showed linear dependence on the concentration, ranging from 1.0 × 10^-8 to 4.0 × 10^-4 M with a limit of detection of 4.1 × 10^-10. The NFN level in the studied tablet brands was in the range of 90.30-103.3% of their labeled values. Recovery results in tablet and urine samples ranged from 98.35 to 101.20% and 97.75 to 99.60%, respectively, and interference recovery results were less than 2.13% error in the presence of ampicillin, chloroquine phosphate, and cloxacillin. The present method had a better performance for the determination of NFN in tablet formulations and urine samples as compared with recently reported voltammetric methods due to its requirement of a simple electrode modification step, which provides the least limit of detection and a reasonably wider linear dynamic range.