中国仓鼠卵巢细胞
生物过程
HEK 293细胞
重组DNA
细胞培养
生物
转染
细胞生物学
转基因
病毒学
病毒
胚胎干细胞
维罗细胞
生物技术
基因
生物化学
遗传学
古生物学
作者
Nuri ERGEN,Halil TÜFEKÇİ
出处
期刊:Journal of experimental and clinical medicine
[Journal of Experimental and Clinical Medicine]
日期:2022-08-30
卷期号:39 (3): 884-892
标识
DOI:10.52142/omujecm.39.3.55
摘要
A various number of expressions and host systems are used in biologics manufacturing. The most commonly preferred systems are based on bacteria, yeast, mammalian cells, insect cells, and transgenic animals. A wide range of molecules, including insulin, mAbs, vaccines, and recombinant proteins, are produced using different host systems. Because of several reasons impacting the product quality and yield, mammalian cells are utilized. Moreover, mammalian cells are generally used in virus-based vaccine manufacturing. Chinese Hamster Ovary (CHO) is the most widely used cell line for high yield stable recombinant protein production, while Human Embryonic Kidney (HEK) is favoured for transient transfection low yield protein manufacturing, viral-based vaccine and gene and cell therapy-related vector production. Other mammalian cell lines such as NSO, Sp2.0, Vero, MRC-5 and PerC.6 are also used in both recombinant protein and virus productions. Multiple modifications are carried out on industrial cell lines to make them more suitable for high yield and high-quality protein production. Thanks to these alterations, high productivity and quality levels are achieved in the biotechnology industry.
科研通智能强力驱动
Strongly Powered by AbleSci AI