氧化应激
黑腹果蝇
生物
氧化磷酸化
细胞生物学
细胞凋亡
抗氧化剂
生物化学
男科
医学
基因
作者
Rener Mateus Francisco Duarte,Serena Mares Malta,Fernanda Naves Araújo do Prado Mascarenhas,Vinícius Prado Bittar,A. L. C. C. Borges,Renata Roland Teixeira,Renata Graciele Zanon,Carlos Ueira Vieira,Foued Salmen Espíndola
标识
DOI:10.1016/j.etap.2024.104388
摘要
Embryonic development is exceptionally susceptible to pathogenic, chemistry and mechanical stressors as they can disrupt homeostasis, causing damage and impacted viability. Oxidative stress has the capacity to induce alterations and reshape the environment. However, the specific impacts of these oxidative stress-induced damages in the gastrointestinal tract of Drosophila melanogaster larvae have been minimally explored. This study used 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH), a free radical generator, to investigate oxidative stress effects on Drosophila embryo development. The results showed that exposing Drosophila eggs to 30 mM AAPH during 1st instar larva, 2nd instar larva and 3rd instar larva stages significantly reduced hatching rates and pupal generation. It increased the activity of antioxidant enzymes and increased oxidative damage to proteins and MDA content, indicating severe oxidative stress. Morphological changes in 3rd individuals included decreased brush borders in enterocytes and reduced lipid vacuoles in trophocytes, essential fat bodies for insect metabolism. Immunostaining revealed elevated cleaved caspase 3, an apoptosis marker. This evidence validates the impact of oxidative stress toxicity and cell apoptosis following exposure, offering insights into comprehending the chemically induced effects of oxidative stress by AAPH on animal development.
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