生物
PI3K/AKT/mTOR通路
细胞生物学
癌细胞
癌症研究
先天免疫系统
癌症
信号转导
自然杀伤细胞
细胞毒性
免疫学
免疫系统
生物化学
遗传学
体外
作者
Zuoping Li,Jiaru Zhang,Shiwan You,Jing Zhang,Yuling Zhang,Zubair Akram,Shiguo Sun
标识
DOI:10.1016/j.yexcr.2024.113933
摘要
Natural killer (NK) cells are triggered by the innate immune response in the tumor microenvironment. The extensive set of stimulating and inhibiting receptors mediates the target recognition of NK cells, and controls the strength of the effector reaction countering specific targeted cells. Yet, lacking major MHC (histocompatibility complex) MICA/B class I chain-related proteins on the membrane of tumor cells results in the failure of NK cell recognition and ability to resist NK cell destruction. Searching databases and molecular docking suggested that in cervical cancer, pterostilbene (3,5-dimethoxy-40-hydroxystilbene; PTS) in Vaccinium corymbosum extract could constrain PI3K/AKT signaling and improving the MICA/B expression. In flow cytometry, MTT assay, viability/cytotoxicity assay, and colony development assays, PTS reduced the development of cervical cancer cells and increased apoptosis. The quantitative real-time PCR (qRT-PCR) and a Western blot indicate that PTS controlled the cytolytic action of NK cells in tumor cells via increasing the MICA/B expression, thus modifying the anti-tumor immune response in cervical cancer.
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