离体
示踪剂
协议(科学)
体外
医学
化学
病理
物理
核物理学
生物化学
替代医学
作者
Jianglu Xiang,Yiyuan Xia,Shilin Luo,Zhentao Zhang,Keqiang Ye
出处
期刊:STAR protocols
[Elsevier]
日期:2024-03-01
卷期号:5 (1): 102788-102788
标识
DOI:10.1016/j.xpro.2023.102788
摘要
Alpha-synuclein (α-Syn) positron emission tomography (PET) imaging is a valuable approach for diagnosing and monitoring synucleinopathies-related diseases, such as Parkinson disease. Here, we present a protocol for screening potential α-Syn PET tracers using in vitro and ex vivo approaches. We describe steps for employing recombinant pre-formed fibrils and conducting screening procedures on neuronal models, mouse models, and patients' brain tissue sections to assess the specificity and selectivity of the candidate compounds. For complete details on the use and execution of this protocol, please refer to Xiang et al. (2023).1
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