Glycosylation of flavonoids by sucrose- and starch-utilizing glycoside hydrolases: A practical approach to enhance glycodiversification

Flavonoids are ubiquitous and diverse in plants and inseparable from the human diet. However, in terms of human health, their further research and application in functional food and pharmaceutical industries are hindered by their low water solubility. Therefore, flavonoid glycosylation has recently attracted research attention because it can modulate the physicochemical and biochemical properties of flavonoids. This review represents a comprehensive overview of the O-glycosylation of flavonoids catalyzed by sucrose- and starch-utilizing glycoside hydrolases (GHs). The characteristics of this feasible biosynthesis approach are systematically summarized, including catalytic mechanism, specificity, reaction conditions, and yields of the enzymatic reaction, as well as the physicochemical properties and bioactivities of the product flavonoid glycosides. The cheap glycosyl donor substrates and high yields undoubtedly make it a practical flavonoid modification approach to enhance glycodiversification.