Conversion of CO 2 into organic acids by engineered autotrophic yeast

衣康酸 原材料 代谢工程 自养 生物反应器 异源的 生化工程 毕赤酵母 商品化学品 制浆造纸工业 发酵 化学 生物技术 食品科学 生物化学 生物 有机化学 细菌 基因 催化作用 聚合物 工程类 遗传学 重组DNA 共聚物
作者
Michael Baumschabl,Özge Ata,Bernd M. Mitic,Lisa Lutz,Thomas Gassler,Christina Troyer,Stephan Hann,Diethard Mattanovich
出处
期刊:Proceedings of the National Academy of Sciences of the United States of America [National Academy of Sciences]
卷期号:119 (47) 被引量:52
标识
DOI:10.1073/pnas.2211827119
摘要

The increase of CO 2 emissions due to human activity is one of the preeminent reasons for the present climate crisis. In addition, considering the increasing demand for renewable resources, the upcycling of CO 2 as a feedstock gains an extensive importance to establish CO 2 -neutral or CO 2 -negative industrial processes independent of agricultural resources. Here we assess whether synthetic autotrophic Komagataella phaffii ( Pichia pastoris ) can be used as a platform for value-added chemicals using CO 2 as a feedstock by integrating the heterologous genes for lactic and itaconic acid synthesis. 13 C labeling experiments proved that the resulting strains are able to produce organic acids via the assimilation of CO 2 as a sole carbon source. Further engineering attempts to prevent the lactic acid consumption increased the titers to 600 mg L −1 , while balancing the expression of key genes and modifying screening conditions led to 2 g L −1 itaconic acid. Bioreactor cultivations suggest that a fine-tuning on CO 2 uptake and oxygen demand of the cells is essential to reach a higher productivity. We believe that through further metabolic and process engineering, the resulting engineered strain can become a promising host for the production of value-added bulk chemicals by microbial assimilation of CO 2 , to support sustainability of industrial bioprocesses.
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