Carbon quantum dots, are characterized by their exceptional fluorescence properties, low toxicity, and broad potential in biological applications and bionanotechnology. In this study, carbon dots derived from Bletilla striata (BS-CDs) were synthesized to investigate their antioxidant activity, stability, and their effects on the growth of mung bean sprouts. The results showed that BS-CDs possess remarkable antioxidant properties and excellent stability. At lower concentrations, BS-CDs significantly promoted plant growth, whereas higher concentrations exerted inhibitory effects. The optimal concentration for growth enhancement was determined to be 0.4 mg/mL (an increase of 36.4% compared to the deionized water control group). These findings highlight the potential of BS-CDs as innovative agricultural supplements, leveraging their antioxidant activity and concentration-dependent effects to improve plant growth.