上睑下垂
纽恩
脊髓损伤
脊髓
污渍
下调和上调
免疫印迹
细胞生物学
神经科学
生物
免疫组织化学
程序性细胞死亡
医学
细胞凋亡
免疫学
基因
生物化学
作者
Chao Liu,Qianliang Wang,Shenye Yuan,Sunao Li,Xueshi Chen,Xinqi Huang,Jun Yan,Haiyan Shan,Mingyang Zhang
标识
DOI:10.1016/j.jchemneu.2022.102229
摘要
Rab7 belongs to the Ras small GTPase superfamily, and abnormal expression of Rab7 can cause neuropathy and lipid metabolism diseases. Studies have shown that Rab7 plays a crucial role in the inner membrane translocase. However, the role of Rab7 in the regulatory mechanisms of cell survival in spinal cord injury remains unknown. We used a rat spinal cord injury (SCI) model to explore the cellular localization and expression of Rab7 after SCI in this study. Western blot analysis showed that Rab7 was expressed in the spinal cord tissue. On the first day, it significantly increased and peaked after SCI on the third day. Furthermore, western blotting also demonstrated that pyroptosis-related protein Gasdermin D (GSDMD), Caspase-1, apoptosis-associated speck-like protein (ASC) expression peaked after the third-day post-injury. Importantly, the immunohistochemistry analysis revealed that Rab7 was completely colocalized with ASC in neurons after SCI. These results suggested that Rab7 was colocalized with NeuN and ASC, involved in the pyroptosis of neurons, and closely related to the spinal cord after injury.
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