奥沙利铂
结直肠癌
癌症研究
小RNA
细胞凋亡
细胞生长
化学
糖酵解
免疫印迹
生物
癌症
医学
内科学
新陈代谢
生物化学
基因
作者
Xiaofeng Qiu,Qingmei Xu,Bingling Liao,Sheng Hu,Ying Zhao,Huijun Zhang
出处
期刊:PubMed
日期:2023-10-01
卷期号:38 (10): 1145-1156
被引量:3
摘要
Colorectal cancer (CRC) is a malignancy that threatens the patient's life. Previous reports showed that circular RNAs (circRNAs) can affect CRC development. Herein, we demonstrated the characters of circular RNA copper chaperone for superoxide dismutase (circ-CCS) in CRC tissues and cells.Circ-CCS, CCS mRNA, microRNA-874-3p (miR-874-3p) and hexokinase 2 (HK2) were indicated by qRT-PCR and western blot in CRC. The cell roles were examined. Additionally, the interaction between miR-874-3p and circ-CCS or HK2 was forecasted by the bioinformatics method and assessed by dual-luciferase reporter assay. Finally, the mouse test was implemented to demonstrate the effect of circ-CCS in vivo.Circ-CCS and HK2 were increased, whereas miR-874-3p was diminished in CRC. Circ-CCS lack subdued the IC₅₀ value of oxaliplatin, cell proliferation, migration, invasion and glycolysis metabolism in CRC cells, while it endorsed cell apoptosis. Furthermore, miR-874-3p was validated as having a tumor repressive effect in CRC cells by restraining HK2. The results also showed that HK2 could regulate the development of CRC. In mechanism, circ-CCS targeted miR-874-3p to control HK2. In addition, circ-CCS knock-down also attenuated tumor growth in mice.Circ-CCS expedited CRC through miR-874-3p/HK2.
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